Acrylamide

Characteristics

Uses

Environmental Transport

Environmental Deposition

Methods for Monitoring in the Environment

Methods for Monitoring Human Exposure

Safeguards Against Acrylamide Exposure


Harmful Effects

Dose Response

Absorption, Distribution and Metabolism

Primary Sites for Toxicity

Biomarkers

Mechanism of Action

Risk Assessment and Management

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Acrylamide Policy

Introduction to Policy

Decision Makers and Stakeholders

Current Policy

Policy Recommendations


References

METHODS FOR MONITORING ACRYLAMIDE IN THE ENVIRONMENT

AIR
Methods for monitoring airborne acrylamide levels in production facilities have been developed my Dow Chemical and modified by the National Institute for Occupational Safety and Health (NIOSH). The method presented by NIOSH involves collecting an air sample in a midget impinger sampling device that is worn by the worker. Distilled water that is completely free of contaminating substances is used as the collection media. A known volume of air is drawn through the impinger and used in the final calculation for concentration. After collecting the sample an aliquot of the collection media is then analyzed using polarographic detection.

FOOD
Due to recent concerns over acrylamide levels in food products, the need for an analytical method to assess these levels is very important. Several key aspects must be considered when developing a method for assessing acrylamide exposure in food. The method must be selective enough to differentiate acrylamide from other possible matrix interferences. The method must be quick enough to allow processing of many samples in a timely manner. The use of automated sampling devices greatly facilitates this.

The first step in preparing a food sample for testing is liquid extraction of the acrylamide from the sample. A solvent is used that is effective in extracting acrylamide from the food sample without co-extracting starch, which can interfere in the analysis. The great water solubility of acrylamide is then taken advantage of by re-extracting the acrylamide from the solvent, resulting in an aqueous solution almost free of any interfering matrix components. The use of filter centrifugation may be used to facilitate this process. The next step is to analyze the sample using high pressure liquid chromatography (HPLC) coupled to a tandem mass spectrometry (MS).

WATER
Methods for monitoring acrylamide in drinking water have not been established. Because no analytical methods are available monitoring for acrylamide is not required. Acrylamide is regulated by requiring the use of a treatment technique that limits its use in drinking water systems. The treatment technique may not exceed the level of .05% acrylamide at 1mg/L.